Yang, L., Gradl, R., Dierolf, M., Möller, W., Kutschke, D., Feuchtinger, A., Hehn, L., Donnelley, M., Günther, B., Achterhold, K., Walch, A., Stoegel, T., Razansky, D., Pfeiffer, F., Morgan, K.S. & Schmid, O. Multimodal precision imaging of pulmonary nanoparticle delivery in mice: dynamics of application, spatial distribution, and dosimetry. Small, 15(49), 1904112 (2019). https://doi.org/10.1002/smll.201904112
Purpose: The multimodal imaging approaches provide unprecedented concomitant insights into dose, distribution, and mechanism of pulmonary delivery of drugs at high temporal and spatial resolution. In vivo PB‐PCXI allows for noninvasive time‐resolved monitoring of the dynamic process of drug/nanoparticle delivery, while fluorescence‐based ex vivo imaging (IVIS and tissue‐cleared LSFM) offers quantitative dosimetry and accurate 3D localization of fluorescent tracers (here NPs) with cellular resolution at one time point.
Figure: Leveraging multimodal imaging for a comprehensive understanding of the characteristics and delivery mechanism of ventilator‐assisted aerosol inhalation.